CARD8 Knockout cell line (Jurkat, Clone E6-1)

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CARD8 Knockout cell line (Jurkat, Clone E6-1) Order

CARD8 Knockout cell line (Jurkat, Clone E6-1)
Catalog#
YKO-H771
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
CARD8 Knockout cell line (Jurkat, Clone E6-1)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
CARD8 Knockout cell line（Jurkat, Clone E6-1）
Cell Morphology
Lymphocyte-like,suspension
Passage ratio
1:3-1:5
Species
Human
Gene Name
CARD8
Gene ID
22900
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%RPMI-1640+10%FBS
Accession
CVCL_E0WG(Cellosaurus)
ExPASy Cellosaurus Database
Is a valuable online resource that compiles detailed information on over 100,000 cell lines from reputable cell banks such as ATCC, ECACC, and DSMZ. The database provides information including descriptions, origins, and characteristics of the cell lines, with broad reference value and scientific reliability. Additionally, the database provides information on cell line authentication, STR profiles, and potential contamination risks, helping researchers ensure the trustworthiness of the cell lines used in their experiments.
Target region
Knockout region: Exon 8 (based on transcript CARD8-231)
Validation: PCR+Sanger Sequencing
CARD8 Knockout cell line (Jurkat, Clone E6-1) Target region
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:Jurkat, Clone E6-1		 STR Info (Cell bank)
Cell Line:Jurkat, Clone E6-1
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 9999
D13S317 812812
D7S820 812812
D16S539 11111111
VWA 18181818
TH01 69.369.3
AMEL XYXY
TPOX 810810
CSF1PO 11121112
D12S391 2223
FGA 2021
D2S1338 1923
D21S11 31.233.2
D18S51 132122.0
D8S1179 1314
D3S1358 1515
D6S1043 1111
PENTAE 1012
D19S433 1415.2
PENTAD 1113
D1S1656 1617
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the CARD8 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Jurkat, Clone E6-1 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, CARD8 gene knockout CARD8 Knockout cell line（Jurkat, Clone E6-1） will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
CARD8 Knockout cell line (Jurkat, Clone E6-1)
CARD8 Knockout cell line (Jurkat, Clone E6-1)
Catalog#
YKO-H771
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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